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The quantification limits of the assay for both Ureaplasma species ranged from 2.106 to 2.101 copy numbers per PCR.
The larger venom amounts are overwhelming quantities designed to test the limits of the assay for arthropod venom cross-reactivity.
IL-17A and IL-17F levels fell outside the detection limits of the assay used but have been provided in Fig. S2 of the Supplementary Information, presented using fluorescence intensity rather than pg/ml.
The detection limits of the assay were about 2 40 cfu/g for artificially contaminated meat products after 8 h enrichment period at 30 °C.
Detection limits of the assay were ≤160 and ≥600 FORT units.
Levels of IL-1A, IL-10, and IL-12 were below detection limits of the assay (12.0 pg/ml).
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The detection limits of the assays were 0.2 ng/mL and 2 pg/mL, respectively.
The dashed line represents the detection limit of the assay.
The lower detection limit of the assay is 0.1 EU/mL.
Detection limit of the assay is 0.02 nmol/L.
Only cytokines with robust signals above the detection limit of the assay were included in the analysis.
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