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Low and high m/z limits of mass spectrometer were set at 50 and 400 amu, respectively.
Even though much development has been achieved in this field, many materials are not applicable in the real field due to the limits of mass production and price.
For the frustrated people here, the limits of mass transit restrict the boundaries of possibility, reinforcing poverty and a nebulous sense of futility.
"What's more important is actually making clear what the limits of mass surveillance ought to be and then having an inspector general enforce that on the back end," said Harley Geiger, senior counsel and deputy director of the Freedom, Security and Surveillance Project at the Center for Democracy and Technology.
Among the available technologies in proteomics, RPPM represents one of the most flexible and robust technology, providing quantitative broad-scale measurement of hundreds of phosphoproteins, even low abundance signaling molecules that are below the detection limits of mass spectrometry.
Protein quantification was done with an SILAC workflow implemented in the Proteome Discover using the following parameters: Limits of mass and retention differences between heavy and light pairs should be smaller than 4 ppm and 0.2 min, respectively; only unique peptides were used for quantification; and protein ratio was determined using the median of the corresponding peptide ratios.
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On the other hand, the limit of mass transfer rate can be raised in the various wavelengths of photo-assistance, which helps replenish ions to the substrate surface.
The limit of mass transfer rate is higher than the deposition rate in the various wavelengths of photo-assistance, creating a smoother surface, as shown in Figure 3a, b, c, d, e. Figure 4 shows the linear sweep voltammogram with higher Cu concentration which helps to verify the limit of mass transport effect.
The factors pointed out as important on the robustness of the system were photomultiplier power for quantitative analysis and lower limit of mass scanning range for qualitative analysis.
We focus storage of large molecular/cluster ions taking advantage of the fact that the electrostatic ion storage ring is free from the limit of mass of circulating ions.
As calculated from the detection limit of mass spectrometry and the amount of cells used in this study, the least abundant surface protein identified here should be presented on each cell at the level of hundreds of copies [37].
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