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Modeling of the climatic correlates of H. binoei distributions and biophysical modeling of limits for successful egg development and minimal foraging time strengthen our phylogeographic scenario.
Predicted correlative distribution models for parthenogenetic H. binoei and biophysical predictions for the temperature limits for successful egg developments and minimal foraging activity are shown in Figure 4. Three scenarios are presented, with mean annual rainfall reductions of 1/2, 1/3, and 1/4 (all with a 9°C average temperature reduction).
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Using our data, it is possible to estimate the storage time to reach sterility or the required lower limit for successful seed inoculation of 1000 CFU/seed.
Kearney and Porter [25] showed that in many places the southern limit of the range of the EA6 sexual chromosome race (the most southerly distributed chromosome race) coincides very closely with the thermal limit for successful egg development; similar climatic constraints on the 3N1 southern distribution are likely to be in place.
Post-ovulation oocyte ageing in the ovary of fish is one of the limiting factors for successful artificial fertilization (Bahre Kazemi et al. 2010).
The implantation process is considered the most relevant limiting factor for successful pregnancy.
Another limiting factor for successful genome-wide studies is the accuracy and density of the genotype information.
We argued that if indeed EPI cell number is the primary limiting factor for successful development, then half-embryo development should be rescued by expanding the EPI population.
These data further demonstrated asymmetric primer concentrations in the bridge PCR to be a rate-limiting step for successful production of fused sequences.
This study indicates that constraints of host physiology or genetics have limited opportunities for successful horizontal transfers during reproductive parasite evolution.
The limiting factor for successful hematopoietic stem cell transplantation (HSCT) is graft-versus-host disease (GvHD), a post-transplant disorder that results from immune-mediated attack of recipient tissue by donor T cells contained in the transplant.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com