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The vast majority of experimental studies are limited to replicating only parts of the physical mechanisms and couplings because of the difficulty in imposing the complete transient, hypersonic environment.
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The population is limited to 400 replicating cells: when the population size drops below 400 a random cell, that has divided more than 24 hours (set to be cell doubling time) prior to present event, is picked and is allowed to divide.
While many other RRBS studies were limited to two replicates per tissue we analyzed 5 biological replicates demonstrating very high reproducibility.
We were logistically limited to 15 replicates for E3, because distances of 90 + meters in all other locations were situated in topographically steep or rocky areas where the tree community was markedly different in structure and composition, and we wanted to control for variation in elevation and topography.
The validation for milk was limited to seven replicate measurements at MRL on 1 day.
Although initially believed that HRV infection was limited to the upper airways, replicating virus was found in ciliated epithelial cells of the lower respiratory tract.
These findings show that an attenuated virus retaining limited ability to replicate nonetheless can effectively mobilize CD8+ cellular immunity and will be important for the design of picornavirus vectors used as immunotherapy in clinical settings.
As the platelet is anucleated, it has limited ability to replicate new proteins or enzymes, thus resulting in irreversible inhibition of the enzyme for the life span of the platelet, namely 7 10 days.
The research described herein is limited to the normalization of replicate microarrays.
Although RNAseq analysis of infected material was limited to a single biological replicate, we used the DESeq approach [ 35] to assess enrichment as this package is suited for working with partial replicates [ 36].
The heuristic search used tree bisection reconnection (TBR) limited to 1000 rearrangements per bootstrap replicate due to computational constraints.
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