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Although a number of significant improvements have been made, the methodology still has a number of inherent technical and physical limitations, such as the limited throughput of samples and the requirement for cloning and amplification of the DNA prior to sequencing.
Given the fact that data is aggregated for multiple end users at each router, the limited throughput of 128 kbps may not fulfill the requirements of any application.
However, the limited throughput of the SPR-based assay prevents it from being used as the primary assay in high-throughput applications 7, 104.
Considering the large number of CNVs reported (>6000 entries in the database of human variation) the number of validated CNVs using independent quantification methods such as RT-qPCR or FISH is still proportionally very small due to the time consuming or limited throughput of single locus analysis.
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Although the plasma extraction device exhibited limited throughput because of the use of a passive flow handling method, modifying the design and integrating sensors would make our device suitable for use in simple hematological tests of plasma.
To deal with limited throughput backhaul, most of the works in the literature focus on the simpler clustering approach where the BSs are organized in clusters and joint processing is applied within each cluster by sharing the whole data to be sent among all the BSs of the cluster.
Because of the limited throughput and high false-positive rate of the two methods mentioned above, microarray was applied to several species to obtain large-scale transcriptional regulation information during organ abscission in apples [ 22, 25], citrus (Citrus clementina) [ 26] and tomato (Solanum lycopersicum) [ 27].
While it is clear that cloud computing has great potential for research purposes, for small labs and clinical applications using benchtop genome sequencers of limited throughput, like MiSeq, PGM, and Proton, cloud computing does have some practical utility.
Although the use of these markers is not without limitations (such as the need for complex and nonstandardized sample pretreatment methods in order to obtain concentrates before laboratory analysis and the uncertain validity for some of the assays, limited throughput development, elevated cost, low sensitivity, and results reflecting only short-term exposure evaluations).
These designs are prone to limitations such as the exertion of physical stress on the worms and limited throughput.
The main drawback of these methods is the limited throughput due to required time-consuming steps.
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