It also possesses utility within clinical settings where only limited quantities of samples may be available (e.g. microdissected material) or when minimally invasive procedures are performed (e.g. biopsied specimens).
However, we were unable to detect atrogin-1 and MuRF protein using commercial antibodies (Abcam) and limited quantities of sample precluded direct measurements of Ub-proteasome activity.
The recent application of single-cell RNA-Seq has allowed profiling of samples with limited quantities of sample such as embryonic development, but this technique did not discriminate strand-specific transcripts and did not detect 5' ends of transcripts longer than 3 kb which would hinder analysis of alternative promoter usage [ 16, 17].
In IHC, multiplex staining is growing need within limited quantity of clinical samples.
Due to the limited quantity of serum samples we had to focus on a panel of crucial mediators.
Due to limited quantity of mitochondria samples, no technical replication was performed and so we set the criteria of fold change >1.4 or <0.72 between PBC mouse model and control for identifying differentially expressed proteins, more stringent than that accepted by several previous research [ 18- 22], in which the cutoff were at >1.2 or <0.82.
We carried out a modification of SAGE methodology (microSAGE), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions.
Such samples often contain a limited quantity of nucleic acids of varying quality, complicating library construction.
Since our experiments have been performed with fresh breast tumor samples which provide only limited quantity of cells after disaggregation we were not able to apply alternative test systems such as MTT or XTT assay to validate the outcome.
Because of the limited quantity of serum specimens, the complete array of virologic tests was not performed in all samples, but available serologic data demonstrated acute PUUV infections in all patients (Table 2).
Due to the limited quantity of some of the field sera, only the two HI tests (HI-HRBC and HI-CRBC) could be performed on these samples.
