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It also possesses utility within clinical settings where only limited quantities of samples may be available (e.g. microdissected material) or when minimally invasive procedures are performed (e.g. biopsied specimens).
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However, we were unable to detect atrogin-1 and MuRF protein using commercial antibodies (Abcam) and limited quantities of sample precluded direct measurements of Ub-proteasome activity.
The recent application of single-cell RNA-Seq has allowed profiling of samples with limited quantities of sample such as embryonic development, but this technique did not discriminate strand-specific transcripts and did not detect 5' ends of transcripts longer than 3 kb which would hinder analysis of alternative promoter usage [ 16, 17].
We carried out a modification of SAGE methodology (microSAGE), useful for the analysis of limited quantities of tissue samples, on normal human cervical tissue obtained from a donor without histopathological lesions.
Because of limited quantities of jewelry items, samples selected for saline extraction were, in general, subsequently tested by the 0.07 M HCl extraction.
Because of limited quantities of the plasma sample, we were unable to sequence the complete genome of the plasma-derived MLB2.
In IHC, multiplex staining is growing need within limited quantity of clinical samples.
Due to the limited quantity of serum samples we had to focus on a panel of crucial mediators.
Due to limited quantity of mitochondria samples, no technical replication was performed and so we set the criteria of fold change >1.4 or <0.72 between PBC mouse model and control for identifying differentially expressed proteins, more stringent than that accepted by several previous research [ 18- 22], in which the cutoff were at >1.2 or <0.82.
Because environmental samples may contain limited quantities of intact pathogen DNA, we expect that sample material will need to be amplified to generate the amount of target DNA required to produce a detectable signal on the array.
In many instances only limited quantities of genomic DNA are available from clinical samples for genetic testing.
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