The limited biomass obtainable from these plants necessitated the pooling of leaf material from all transgenic lines.
One limitation of a large study is that limited measures were obtainable; e.g., measures of maternal body fat and its distribution are not available.
Despite the progresses made in recent years, mixing in microfluidics still remains as a challenge, mostly arising from a low Reynolds number (Re) by the small dimensions of microchannels and the limited range of obtainable velocities in its operation.
While this is not generally an issue in humans, the limited body fat obtainable in rabbits may impact the quantity of ASC.
Thereby, a fiber probe with 5 mm source-detector distance and 0 mm fiber protrusion still has a feasible geometry, despite the limited spectral range obtainable in one acquisition for the measured tumor phantoms.
Sometimes however this technique is not feasible for many reasons, like the limited amount of obtainable graft tissue in case of large and extended loss of substance, the unavailability of the proximal nerve stump, and the morbidity at the donor site.
In our ICU, only limited biochemical parameters were obtainable; however, close monitoring of the above-mentioned parameters helped us to make early diagnosis and treatment assessments.
The trade-off is the loss of any information on spatial distribution of fluorescence lifetime within the cells and the limited number of photons obtainable from each cell due to their finite dwell time in the interrogation space.
HMSCs isolated from the bone marrow have several limitations, however, the most paramount is the limited number of cells easily obtainable.
Because of the reliance on de-identified medical records in this study, there were a limited number of potential confounders obtainable for analysis: sex, age, socio-economic status, and ethnicity.
