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To address the cause of the initial decrease of light emission in 4T1-luc2-1A4 clone, we performed limited dilution culture in 96-well plates.
Figure S1: RNA/Tau fibril interactions at elevated salt concentrations and stability of Tau fibrils upon limited dilution.
A431 clones with transduction of hDsg3.myc10 were obtained using the limited dilution assay.
We also estimated several RGK1 sub-clones using the limited dilution method.
Cells were cloned by limited dilution and a clone showing no MHC I surface expression was selected for this study.
Cells were selected with puromycin (2 μg ml−1, Sigma, St Louis, MO) and drug-resistant cells were subcloned by limited dilution in 96-well plates.
Limited dilution was used in the cell colonies that formed 24 48 h after transfection, and the cell concentration was approximately 500 cells/dish (10 cm2).
His contributions; cloning by limited dilution, and massively parallel DNA sequencing, are the basis of all subsequent high-speed sequencing methods.
This is a conservative estimate because several factors not accounted for in our analysis (industrial pollution, eutrophication, seasonal discharge cycles and smaller streams with limited dilution capacity) would further increase these numbers4,6.
Clonal sublines expressing different levels of NOS II were then established using a limited dilution technique.
His goal is to continue to grow the company with limited dilution to shareholders, a message that apparently has been well-received.
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