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Many of the challenges of diagnosing tuberculosis in the eye are encountered such as limited amount of specimen available for testing, lack of laboratory investigation, poor yield from intraocular specimen, and the absence of uniformity in its diagnostic criteria [ 7].
Because additional blood sampling was not easily accepted in many countries including Viet Nam, BIBD was attempted to obtain analytical information from small volume of plasma samples in this study: Of four possible combinations of harvester and ELISA operator, two combinations were cyclically chosen, using the limited amount of specimen.
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This approach has been shown to enhance the capacity to analyze complex protein mixtures from a limited amount of a specimen [11].
Thus, miRNA-based procedures may not be suitable if a limited amount of plasma/serum specimens is available.
Technical constraints such as the limited amount of clinical specimens, the use of different screening methods, and the need to sequence an unknown target of extreme variability might have limited the representativeness of our sequence collection.
Due to the limited amount of primary glioma specimens, the cell cycle analyses were executed on primary tumor spheres from three independent tumor samples.
ISH is an ideal assay for validating miRNA expression at single-cell resolution using a limited amount of a clinical tissue specimen.
Nevertheless, it is impossible to perform multiple tests on very limited amounts of biopsy specimen that obtained in needle biopsy.
Liver biopsy caries a significant morbidity and even mortality risk and it is impossible to perform multiple tests with current techniques on very limited amounts of biopsy specimen that obtained in needle biopsy.
As already noted, the difficult taxonomy of the group, and the limited amount of available data and specimens, makes difficult the rigorous assessment of the partition of the measured variation among and within species.
IgE binding of patients' IgE was analyzed by dot-blotting for limited amounts of serum specimens as described previously (Wakasa et al., [2011a]).
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