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Due to the limited amount of samples available, analysis was limited to 11 tumour samples disclosing low levels of pAMPK expression and to additional three samples with high levels of pAMPK expression.
A limited amount of samples with thousands of features, give rise to known issues, as data overfitting and multicollinearity.
However, we did not sequence PCR products amplified from rodents at other study sites because of a limited amount of samples.
Unfortunately, the expression of genes linked to aberrantly methylated DMRs could not be analyzed due to poor RNA quality, which was probably due to effects of chemotherapy and a limited amount of samples.
Such a change in repeat length could well occur before the first passage or during very early passages, during which it is impossible to do southern blot analysis to measure the repeat length due to limited amount of samples.
Nevertheless, traditional MHC genotyping approaches may remain an alternative to consider when a limited amount of samples is to be genotyped, as NGS is still expensive and many research groups do not have direct access to these technologies.
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Due to the limited amount of sample available, only single chips of Alais, Y-82162 and Y-980115 were analysed.
This method predicts pixel values for a raster layer from a limited amount of sampled data points.
Number of measured particles was sufficient (over 300) in all studied samples with the exception in nPSi_480 where 75 particles were measured because of the limited amount of sample (Additional file 1: Table S2).
Reverse phase protein microarrays (RPMA) are a technology platform designed for quantitative, multiplexed analysis of specific phosphorylated, cleaved, or total (phosphorylated and non-phosphorylated) forms of cellular proteins from a limited amount of sample.
The quantitative disagreement of the two distributions observed in this figure could be caused by that the size distribution from TEM images being slightly modified because the present observations were based on a limited amount of sample and observed TEM images were random but in small numbers (here n = 114).
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