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This review will summarize current approaches and limitations to isolate PSC from adult tissues or, alternatively, to generate it by nuclear reprogramming from already differentiated somatic cells.
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However, the methodology does have several important limitations including the inability to isolate by culture.
Moreover, from the perspective of theft reduction, an additional limitation is the inability to isolate the degree to which theft is a source of shrinkage, as opposed to other types of crime (such as fraud) and administrative errors.
This can be considered a limitation in the attempt to isolate the impact of the intervention.
Although it was confirmed that Nav1.8 was both expressed on small and medium diameter L4 DRG neurons in different proportions (83.2% of all small diameter cells <700 μm, 17.2% of all medium-to-large diameter cells >700 μm, n=3), it is still a limitation that we failed to isolate the small-sized DRG neurons for the present study.
Highly responsive pen input devices seem critical for user satisfaction not only for sketching, but also for image sorting; the effects of large display spaces are difficult to isolate from the limitations of input device.
Although usually effective, limitations with iPCR hinder its ability to isolate flanking genomic DNA in complex genomic loci, such as those that contain natural transposons.
It should be noted, however, that due to limitations in SPECT resolution, it is impractical to isolate uptake in the putamen from that of the adjacent globus pallidus.
To address these limitations, we introduce a measurement strategy designed to isolate the presumed genetic and sociocultural dimensions of race.
While it is almost certain that a large degree of this trait change does indeed have a genetic basis, probabilistic maturation reaction norms have also been found to have limitations with respect to isolating the various genetic contributions to overall trait change (Kinnison et al. 2011; Uusi-Heikkila et al. 2011).
The strategy adopted in this study to isolate tumour-specific genes has certain limitations.
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