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However, conventional IHC has been faced with several practical limitations to examine tens of biomarkers in clinics: time, labor, diagnostic expense, and the amount of tissues required.
Although the uptake activities were tested in yeast inactivated endogenous nucleoside transporter, FUI1, it has certain limitations to examine the role of mammalian plasma membrane transporter because of different membrane structure, intracellular kinases, and posttranslational modification such as glycosylation which could alter the transporting activity of ENT1.
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One approach that may overcome such limitations is to examine both serum and tissue by detecting and identifying differentially expressed proteins in tissue (using mass spectrometry-based approaches) and then quantifying these in serum with antibody-based tests.
One way around these limitations is to examine and analyze gene expression from many individual genotypes that each contains multiple mutations and to study the covariance in phenotypes and multiple gene expression levels to hypothesize which of the gene expression changes might cause phenotypic deviations [ 38- 41].
Here we present a lab that circumvents both of these limitations, allowing students to examine how selection imposed by small-mouthed predators can favor the evolution of simple multicellularity.
We emphasize that this meta-analysis has limitations and serves to examine published data to generate hypotheses.
It is therefore important not only to study the residents' limitations but also to examine their resources and strengths in relation to coping with loss and to study why older people may manage well despite impaired physical capacity and adversity.
Even with these limitations, the ability to examine the prevalence of established breast cancer risk factors from two population-based sources allowed us to compare the prevalence of breast cancer risk factors in SFBA non-Hispanic white women with similar women in the rest of California.
Data limitations allowed us to examine only the impact of the introduction of Quebec's program (Programme québécois de dépistage du cancer du sein) in 1998, by comparing the change in screening rates at age 50 before the program was in place to the change at age 50 after implementation.
In general, the loss of some patients from an original birth cohort is a limitation to study designs examining variants in severe versus mild forms of the disease.
The literature regarding the burden and risk factors in the Pacific region remains largely limited to descriptive studies in PNG with important limitations in their ability to examine the population-based burden of road crashes and investigate related risk factors.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com