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Thus, the effective use of NGS requires careful assessment of technologies, assay limitations, template requirements, and the research and clinical questions under consideration.
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This combination of ultraclean consumables and reagents, along with improvements incorporated into iMDA provides the opportunity for specific detection and genomic sequence analysis from samples that were previous not possible due to template limitation.
Deformable templates are proposed to cope with the limitations of fixed template matching.
To overcome some limitations of fully organic polyHIPEs hybrid organic inorganic templated porous polymers are particular attractive.
To overcome this limitation, we developed an innovative validation strategy, which takes advantage of synthetic gene templates to establish the limit of detection (LOD), for every target on the microarray for which genomic templates are not available.
However, AppArchitect is one of the first services to offer web-based tools without any of the limitations of a template-based system.
The biggest limitation of this template is the absence of anatomical lateralization of some regions, such as leftward asymmetry of the planum temporale for the vast majority of right-handers [ 46].
A limitation of the template technique is that it may not be applicable in patients with a bilateral fracture or patients with a history of a distal radial fracture or deformity on the other side.
Although 'brute force' DNA genotyping by dideoxy sequencing has been the method of choice for de novo SNP detection, it suffers from difficulty of DNA template amplification, limitations of PCR multiplexing per reaction, and increasing equipment costs.
The limitations of the existing templates involve internal and external validity, and none of them have been tested for feasibility in real-life incidents.
Because SeqZip does not include an RT step and is therefore not subject to RT processivity and template-switching limitations, it can be used to assess intramolecular connectivity between regions separated by thousands of nucleotides.
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