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This is of special importance for pathogenicity related genes that are currently underrepresented in our predicted network due to the limitations (quantity, coverage and divergence) of the currently available gene expression data.
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The approach is used under certain limitations including quantity discounts, capacity, and budget to specify the best suppliers and put the optimum order quantities among them.
Benton (1991) applied Lagrange relaxation to develop a non-linear program for supplier selection under various conditions including multiple suppliers, multiple items, resource limitations and quantity discount.
The bacterial load estimates have limitations: The quantity of material obtained on the swab was assumed to be equal for all swabs; DNA extraction efficiency was considered to be 100%; and molecular weight calculations were based on estimates of GC content.
Finally, we recommend using MDA in order to overcome limitations of DNA quantity (when the amount of DNA is limited) or when only poor-quality DNA samples are available (severely-degraded samples, to increase the number of typed loci).
Re the May 12 Op-Ed article by Frank McKenna, the Canadian ambassador to the United States, about North Dakota's Devils Lake project: The permit awarded for the project by the North Dakota Department of Health, under the authority of the federal Environmental Protection Agency, places strict limitations on quality, quantity and timing of water discharge.
Limitations in the quantity of enzyme produced by an organism compels, cloning and over expression of microbial enzymes regularly.
Because of limitations in sample quantity, samples from each time point per treatment were pooled (n = 1 3 per treatment and time point) for analysis.
Due to length limitations and the quantity of the results/tests performed, the results of the above diagnostic test cannot be presented.
DOA estimation algorithms based on SSR have several advantages over conventional methods, including increased resolution, better robustness to noise, limitations in data quantity, and correlated sources, as well as not requiring an accurate initialization [7].
Design wind loads are partly based on extreme value analyses of historical wind data, and limitations on the quantity and spatial resolution of wind data pose a significant challenge in such analyses.
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