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Nevertheless, it is important to note that bisulfite pyrosequencing has limitations quantifying methylation, as methylation is averaged at each CpG site and the amount of templates showing methylation cannot be deduced [ 14].
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Even Sociometric Solutions Waberr admits the limitations of quantifying everything.
In order to overcome methodological limitations for quantifying the impacts of emission abatement measures, economic evaluation techniques or even Integrated Assessment Methodologies (IAM) have been developed.
Viewshed analysis remains one of the most popular GIS tools for assessing visibility, despite the recognition of several limitations when quantifying visibility from a human perspective.
Although the VNOS proved its reliability and validity (Lederman et al. 2002), it has limitations for quantifying individuals' NOS profiles as well as for administering in a large classroom, especially, in this case, in a required general course for non-major students.
This may be due to the limitations of quantifying physical activity by questionnaire.
However, these methods are time consuming and labor intensive and have inherent limitations in quantifying viral concentration in a large number of clinical samples.
Is any of the difference in nascent RNA and mRNA levels from intrinsic biological variation or from the limitations of quantifying these molecules?
Areas of demarcated opacities and well-defined hypoplastic lesions were measured accurately, but there were limitations in quantifying diffuse opacities and generalised pitting.
C-labeled carbon nanotubes were developed for these experiments to overcome significant previous limitations for quantifying nanotube materials in environmental and biological media.
For instance, binary choice tests have revealed that aliphatic acetates from the preputial glands of male rats are attractive to females, but sexual dimorphism in these compounds has not been demonstrated in chemical analysis (Gawienowski 1977; Stacewicz-Sapuntzakis and Gawienowski 1977), probably due to technological limitations in quantifying the compounds.
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