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Inherent limitations on sequence alignment of short reads prohibit the detection of larger indels and, therefore, many potential disease-causing copy number variations (CNVs) are not accessible from TR data.
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Biological mosaicism and technological limitations on sequencing therefore prevent rigorous copy-number analyses as well as reliable junctional sequence data.
Due to limitation on sequence read length which is too small to span full-length retrotransposons, we were unable to identify these full-length elements.
A reason for the absence of any evolutionary conserved interactions may also be based on limitations of sequence alignment algorithms to accurately detect homologies between eukaryotic and Plasmodium specific proteins.
The small fetal DNA fraction in the maternal blood was the main limitation on sequencing dependent noninvasive prenatal diagnosis.
We chose this method because it is based on thermodynamic considerations of the 30S binding to the mRNA and overcomes the limitations of sequence analysis [ 13].
Despite these limitations on the sequences, for each one of them a large number of low-energy structures can be computed using all known models of the interactions that drive the folding process [8] [11].
Isothermal UV absorbance spectra has a limitation of sequence length.
As a result of this limitation, classification searches on sequence data must be made using each input sequence's forward and reverse-complement effectively doubling the number of classification searches required.
In any event, these results place certain limitations on the possible sequence of events that occur from absorption of a photon to posttranslational modification and ultimate proteolysis of type 1 CRYs.
There are virtually no limitations on the pulse sequences that can be defined.
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