The slow off-rate antagonists that reduce signal and reveal an artifactual non-competitive component to antagonism exhibit another limitation from the reported on assay.
Despite pragmatic limitations on sampling, this type of assay is highly useful for spatial localization of dead cells in CBD biofilms following many antimicrobial treatments (19, 22, 25) as well as for qualitative assays such as the one described here.
The reduced sensitivity of 78.9% (overall) or 87.5% (NPAs) found in this study re-echoes the manufacturer's warning on limitations of the assay.
This dataset also illustrates the limitations of expressing target quantities in units dependent on assay setup and instrument optics.
NPs have been described to interfere with assays, and some reviews report the limitations of certain assay systems [36] and that AuNPs even have the capacity to quench or enhance fluorescence depending on the plasmon field and dipole energy [37].
§ 771.139 Limitations on actions.
§ 17.158 Limitations on assistance.
Limitations on disclosures.
§ 2412.8 Limitations on disclosures.
