Since little is known of the functional role of miRNAs and given the limitations of the programmes that predict miRNA targets, unlike mRNA expression data, cluster analysis is unlikely to provide reliable information on the potential mechanism of action of miRNA in LPS induced inflammatory response.
One explanation of the absence of an effect on overall guideline adherence may be that the time limitation of the programme forced PTs to prioritize, set goals and choose implementation strategies for a limited number of subjects.
Strengths and limitations of the monitoring programme are discussed, and potential future improvements and developments are outlined.
Studies with Personal Advisors [ 36, 37, 39] and the extension of Pathways to existing claimants [ 35, 38] revealed some of the implementation issues and limitations of the existing programme.
However, there are limitations of the current PMTCT programme relate to management, implementation, and prioritization.
Another limitation of the iPlay programme is that less than half of the parents indicated that they received all eight newsletters, as mentioned before.
However, these limitations affect the evaluation of the programme only, but not the quality of care provided at any institution.
From an operational point of view, there are another three very important limitations in the assessment of the programme.
Nevertheless, the lack of further decline in prevalence of STIs from 2001 to 2005 may indicate some limitations in the implementations of the programme.
