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Integrated pH microelectrodes, which overcome the size limitations of reference electrode and counter electrode, are highly desired for in vivo or in situ microscale measurements.
It goes on to identify the limitations of reference genomes and why choosing the closest relative is not always the best option.
The inherent limitations of reference measurements for food and feed safety as well as quality control have necessitated the development of new analytical technologies.
To overcome the limitations of reference independent transcriptome analysis of small numbers of difficult to harvest cells, we developed methods to sample parasite-host interface cells from T. versicolor grown on the distantly related and sequenced model hosts Zea mays (B73) (monocot) and Medicago truncatula (A17) (eudicot) via Laser Pressure Catapult Microdissection (LPCM).
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Other major limitations of reference-based approaches are both that of reference database incompleteness, and that different results can be obtained according to the database size.
These loci have an elevated nonsynonymous substitution rate (Table 3) and appear to have a substantial number of indels in Pinus, although it was not possible in many cases to confidently score indels in these loci due to the inherent limitations of reference-guided assembly of short reads in length variable regions.
Among the existing binning methods, unsupervised methods base the classification on features extracted from reads, and especially taking advantage in case of the limitation of reference database availability.
We also point out that a major limitation of reference-based mapping of NHP data to a human reference will not identify all genes that may be implicated in a particular phenotype.
In addition, these results show the limitations of single reference genome sequences as the only basis for characterization and cloning of resistance genes.
However, researchers and CCRs reported some structural limitations of the reference group meetings and also made positive suggestions how these could be improved for future projects.
Because correlations between the questionnaires and the reference instrument (i.e. the accelerometer) are interpreted as measures of the accuracy of the questionnaires, it is desirable to correct for limitations of the reference instrument that reduce these correlations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com