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I have certain limitations of depth of field with a large camera like this.
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The major limitation of depth of coverage methods arises in duplicated genomic regions, such as segmental duplications or smaller regions of homology between genomic regions.
Importantly, some of the earlier studies based on Sanger sequencing or low depth NGS may underestimate the degree of genomic heterogeneity due to limitations of sequencing depth that preclude the identification of rarer tumour subpopulations, and the study by Xu et al suggests that single cell approaches may be required to assay rare subclones.
Only those contigs formed by five or more ESTs were considered in this analysis to mitigate the limitations of alignment depth in SNP detection, thus resulting in 17,703 usable contigs (36.15% of the total).
Pathological examination has an advantage over fluorescence imaging in that it has no limitation of penetration depth as long as the specimen can be resected.
However, use of optical imaging techniques in large animals is difficult because of depth limitations.
Other restrictions are waving of real-time data transfer and a limitation of operation depths at above the open-hole.
This is caused in part by our current limitation of in-depth knowledge of this process in general and a respective lack of adequate tools and prime candidates suited to reliably reflect alterations of gastrointestinal Se uptake.
In addition, there is a limitation of a range of depth measurement such that the RGB-D sensors can capture the environment from 1 to 4 m.
This study examines whether MID and CP-OCT improve upon the limitations of trying to deduce depth through visual assessment.
We demonstrate how the resolution and imaging depth limitations of nonlinear optical microscopy can be overcome by modulating the spatial overlap between two-color pulses.
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CEO of Professional Science Editing for Scientists @ prosciediting.com