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However, the limitations of cell culture experiments include most importantly the limited transferability of in vitro testing to the in vivo situation.
The rapid advances in stem cell technologies suggest the possibility to overcome the abovementioned limitations of cell replacement therapy.
Limitations of cell formation problem (CFP), as one of important topics in CMS, have led to the introduction of virtual CMS (VCMS).
We discuss advantages and limitations of cell line-based models and highlight models in which tumors spontaneously form as a result of genetic engineering.
Tissue engineering is emerging as a potential therapeutic approach to overcome limitations of cell therapy, like cell retention and survival, as well as to mechanically support the ventricular wall and thereby prevent dilation.
Owing to the technical and practical limitations of cell therapy, cell free conditioned media may represent a potent alternative for therapeutic angiogenesis in ischemic cardiovascular diseases.
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Current limitations of cell-culture hinder progress in understanding the features and dynamics of heterotypic cell communication pathways critical to developing more sophisticated or effective tissue-engineered devices.
Also, easy preparation, long term storage, and avoidance of potential limitations of cell-based treatments shed valuable light on its potential clinical practicality.
Nevertheless, enhanced proliferation and neuronal differentiation rates of ephrin-B3−/− derived NPCs might be one interesting tool for overcoming the limitations of cell-based therapies in cerebral ischemia, such as poor survival and low differentiation rates of transplanted cells.
Understanding cell-cell interactions is important for generating strategies for conditioning cell sheets or other constructs for tissue engineering purposes, or at least to define some limitations of cell-sheet engineering.
Also, based on these findings, the development of cell-free products that can be applied to wounds and improve the quality of healed skin would be a promising issue in this discipline leaving back the hazards and limitations of cell-based treatments.
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