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However, this is not always possible in practice, due to scalability limitations in applications, communication limitations generated by programs during execution, and network restrictions.
In order to eliminate the limitations generated by using E. coli as a single host, shuttle vectors and non- E. coli host systems have been developed.
Compatibility with the PfuTurbo C x Hotstart DNA polymerase relieves the limitations generated by the relatively low fidelity of the Taq enzyme and greatly expands the application range of the cloning method.
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Nevertheless, the motion on the ground requires the implementation of snake-like motion, which is necessary to overcome the limitation generated by the large friction force.
Furthermore, strong diffusion limitation is also generated by the agglomerated amines and coated particles for CO2 from the surface into the bulky amino groups, which would lower the amine efficiency defined as the amounts of adsorbed CO2 molecules for each mole of nitrogen atom.
To some extent this limitation has been generated by a tendency (and perhaps necessity) to only consider a few interactions at a time, rather than the entire spectrum of possible interactions that a single gene may have with the many thousand other genes in the genome.
There are several limitations to the results generated by this review that need to be noted when interpreting the findings.
There are several limitations to the results generated by this review that need to be taken into account when interpreting the clinical relevance of the findings.
Therefore, the cDNA library should have avoided EST limitations that are commonly generated by automatic assemblies of transcripts from different tissues.
There are several inherent limitations of PPI data generated by two-hybrid approaches (both B2H and Y2H), including PPIs that are detected between over-expressed proteins, between fusion proteins, and in a different host (Yeast or E. coli).
The ROR analysis has several limitations: generic under-reporting, over-reporting generated by notoriety bias, dependence on the drug-marketing period (Weber effect), missing or misspelled data (7, 13, 15), and lack of information on patients' habits (smoking) or occupational risks.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com