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In addition, we have identified numerous differentially expressed genes, especially important transcription factor gene wri1 (GenBank KR185335) and studied differential expression of wri1 between control and nitrogen limitation sample, which provided foundation for illuminating the regulation mechanism of lipid biosynthesis induced by nitrogen limitation.
The Illumina Hiseq 2000 platform was applied to D. parva transcriptome sequencing, which produced 26,304,060 (assembled into 60,883 contigs) and 26,797,446 (assembled into 55,236 contigs) high quality reads in samples SCH-5.0 mMA (control sample) and SCH-0.5 mMA (nitrogen limitation sample) respectively.
Only one mPrT study was not biased by co-interventions [ 71] but had other limitation (sample size and baseline imbalances).
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These include insufficient method validation, misinterpretation of raw data, lack of awareness of assay limitations, sample contamination and poor in-house assay design.
One limitation was the limited sample size.
Our study has several limitations including a limited sample size.
The study did have some limitations, sampling more women and city residents — 152 urban districts compared with 112 rural villages.
Our last limitation was sample heterogeneity, which had a significant impact on the construct reliability.
However, due to limitation of sample size, reports on the macro properties of fabricated ceramic structures are still extremely lacking.
Another limitation is sample size.
The authors note limitation of sample size.
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