This is a potentially serious limitation for regions of the genome to which strong candidate genes map and are not properly tagged.
Our focus groups were conducted at only two neonatal intensive care units both located in a single state, opening the possibility of limitations by region, or practice culture.
The limitation of region-of-interest or per channel approaches is that it should be restricted to analysis within a specific group of subjects.
Manual navigation of catheters in the human heart has limitations: some regions are difficult to reach, and compromised catheter positioning may result in insufficient lesion formation.
Although our results do not provide a whole genome view due to the limitations of regions studied and biasness of markers used, our comparative studies have been insightful in understanding the evolutionary process among grasses involving the foxtail millet genome.
Qualitatively, the heat map in Figure 1A indicates that all three nutrient limitations produce regions of strong similarity in transcript abundance pattern; with the G-depleted samples somewhat distinct from the N- and P-depleted samples.
Despite these potential limitations, outlier regions identified in the segregated line are supported by multiple lines of evidence and provide valuable insight for the genetic basis of adaptation to captivity.
Indicators of N richness (e.g., net nitrification, C N ratio, and soil ammonium (NH4+) concentrations) were the strongest predictors of N gas fluxes at the ecosystem scale suggesting that these fluxes are controlled by N limitation in this region.
