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However, this did not exclude limitation of trace elements as inducing signals.
In one batch culture, however, limitation of trace minerals (FeSO4, MnSO4, ZnSO4, CoCl2) was applied and the transient phase between batch and chemostat growth was further disturbed by increasing the concentrations of the trace minerals to those used in other cultures, to shift the limitation from trace minerals to lactose at 0.5 R after the start of continuous medium feeding.
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A diminished N content of K. marxianus was also observed at limitation of growth by trace elements [10,11].
Therefore, more information on advantages and limitations of different tracing methods is needed.
Investigations seeking to identify infected swine were frequently hampered by the limitations of retrospectively tracing suspected swine after exhibition.
ART programs need to maintain current contact information of patients or guardians and should be aware of the limitations of the tracing method used.
We attributed fluctuations in the measured contour lengths mostly to limitations of backbone tracing, rather than actual absolute contour length fluctuations.
To explore the possibility of trace metal limitation in peatlands, we washed 3 peat soils with either PbCl2, to remove available trace metals, or distilled water.
It is known, however, that the enzymes responsible for CH4 production require cobalt, iron and nickel, and there is a growing appreciation for the potential role of trace metal limitation in anaerobic decomposition.
While the cessation of cell growth due to limitation by nitrogen, vitamins or trace elements is unlikely, limitation of other essential nutrients cannot be ruled out.
Thus the levels of trace elements in the cement lines presented in the following are actually a huge underestimate of the real levels of trace elements (see details in " Limitations" section).
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