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To ensure that limitation of quantification to layer IV did not mask any significant results we quantified parvalbumin mRNA expression by RT-PCR with mRNA extracted from all cortical layers.
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There is no evidence of exposure to pesticides or PCBs in the amniotic fluid under the current measurement limitations of quantification.
An experimental limitation in primary hepatocytes concerns the lower limit of quantification of each metabolite.
Lower limit of quantification.
Limit of quantification.
the limit of quantification.
The limit of quantification was 2 nM.
LOQ – limit of quantification.
The methods are critically compared in terms of design limitations, degree of quantification and applicability.
Nevertheless, absolute qPCR has the potential to extend gene expression analysis beyond what is possible with microarray analysis, based upon the innate capability to overcome two of the greatest limitations of microarray quantification, which are limited sensitivity and lack of absolute scale [ 4].
Electrochemical biosensing approaches appeared in the last 5 years for miRNAs determination based on the use of bioreceptors with affinity for RNA duplexes (viral proteins and antibodies) are ideal choices to overcome some limitations of conventional quantification strategies.
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CEO of Professional Science Editing for Scientists @ prosciediting.com