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The main limitation of composition based methods is that the length of fragments may significantly influence their performance.
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(He did not, however, acknowledge the very real limitations of soil composition).
To account for these empirical limitations of cellular composition on the observed expression differences between the X and autosomes, we developed a Bayesian statistical framework based on the relative difference between the spermatogenesis stages (mitosis compared to meiosis) and with the sensitivity to detect down regulation on the X-chromosome during the meiotic phase [ 25].
The third limitation of the existing composition-based approaches is due to the underlying premise of 'one genome-one composition model'.
The second limitation of the existing composition-based approaches pertains to the short lengths of the sequences being generated by next generation sequencing technologies.
The second step also incorporates several new features that attempt to address various limitations of existing composition-based binning approaches.
In addition to overcoming the limitations of existing composition-based approaches, the quick execution time of INDUS confers it a great advantage over other binning methods.
However, INDUS incorporates major modifications in its classification approach in order to overcome the following known limitations of existing composition-based approaches.
However, the studies of detached glycans in complex samples have a common limitation in the undefined changes of composition of the carrier proteins; the changes in protein concentration can contribute significantly to the observed changes in composition of detached N-glycans because glycosylation is protein-specific.
Another limitation of this study concerns the composition of the sample.
Another potential limitation of our study is the composition of our study sample.
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