A major limitation for applying the GFP variants is that the formation of the chromophores strictly requires oxygen, which restricts the use to aerobic systems (Piatkevich and Verkhusha 2011).
There is no fundamental limitation for applying this approach to other complex, multistate membrane protein systems.
However, the model has a few limitations for applied research.
More than half reported respiratory problems.Last month George Pataki, New York's governor, signed laws increasing benefits for thousands of sick first-responders, scrapping the two-year statute of limitations for applying for workers' compensation.
However, EnKF has critical limitations for applying to non-Gaussian distribution fields such as channel reservoirs.
However, we found several limitations for applying the EAF optimization to a realistic large-scale Radio Frequency-Field Programmable Gate Array (RF-FPGA) system.
Uncertainty is considered as one of the most important limitations for applying the results of artificial intelligence techniques (AI) in water quality management to obtain appropriate control strategies.
However, spatial limitations for applying micro-CT-based analyses to large specimens, such as whole vertebral bodies, require using larger scanning and reconstruction voxel sizes.
The limitations for applying the techniques described herein are no longer theoretical or technological, but are now dominated by uncertainty in the physical properties of the flow systems and the lack of data for the historic inputs.
The main limitations for applying this model were the relative lack of region-specific survival data and very few, and probably not always representative, regional cancer incidence data for some developing regions.
The advantages and limitations of applying cocktails for the in vitro activity assessment of major human CYPs, namely, CYP1A2, CYP2A6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1 and subfamily CYP3A, are discussed.
