However, assessing secondary neurosphere formation was precluded because very few spheres were generated in the knockdown groups in the primary assay and the neurospheres had limited viability.
Also, these samples have limited viability.
A key limitation relates to working with a specific functional parameter (Ca2+ influx induced by progesterone) in fresh sperm samples from donors and patients that have limited viability.
Mechanistic studies of active transport are performed preferably in the Caco-2 monolayers, because isolated intestinal enterocytes are fragile and have limited viability.
One of the most common and practical ways for avoiding heat stress in the workplace – working during the coolest (usually early morning) hours – has limited viability for these workplaces since the shift already starts shortly after sunrise.
The major problems can be limited viability, associated with the loss of phase I and phase II biotransformation capacity [ 20]. "Biochips" grow cells in a microfluidic network, allowing a better control of dynamic cultures by continuous feeding of nutrients to cells and waste removal [ 21, 22].
We are fast approaching the absolute limit of viability.
French winemakers are always pushing the limit of viability.
While Ms. Yu has succeeded tiptoeing along such self-imposed limits, these kinds of inhibitions, China scholars say, are likely to limit the viability of Internet businesses -- particularly those that traffic in information or anything politically sensitive.
Gorillas, they suggest, already live on the limit of viability, foraging and eating for 8.8 hours a day, and in extreme conditions increasing this to as much as 10 hours a day.
