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Studying the practice environment in which effectiveness protocols are implemented is necessary to identify the complex challenges that can limit translation of evidence.
This finding is consistent with recent studies indicating that regulated RNA degradation functions to localize mRNAs and to limit translation within axons (Colak et al., 2013).
It was proposed that eIF2B activity does not limit translation and that an alternative yet undiscovered eIF2B function is predominantly responsible for the VWM phenotype.
It is possible that the characteristics that lead to a decrease of translation in stress conditions also limit translation efficiency under control conditions.
Different HEPN domains from TA or other defense systems could have been recruited under this selective pressure owing to their ability to limit translation via RNA degradation or sequestration, thus facilitating stabilization and further development of the eukaryotic intracellular membrane system.
Failure to address a number of specific issues at the time of protocol writing or in the final trial report, such as the PRO hypothesis, timing and mode of assessment and strategies to manage missing data, can undermine the clinical validity of findings and limit translation of these into clinical practice [ 5].
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However, these studies utilised models of simple haemorrhage and may have limited translation into clinical practice.
It is speculated that the absence of a 5′ cap and 3′ poly-(A) tail limits translation initiation.
While there are advantages to currently available small animal models, there are also significant drawbacks that have limited translation of research findings to humans.
Supplementary tRNA is routinely added to reticulocyte lysate with the express purpose of preventing tRNA availability from limiting translation efficiency [37].
Interestingly, restoration of an aromatic ring in the W75F mutant allowed limited translation and resulted in a susceptible-like phenotype (S).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com