We limited this experiment to expression in the head because we wanted to narrow our focus to genes involved in nervous system function.
Thus we limited this experiment to six groups (vs. nine groups in the electrophysiology experiment).
When the rat did not show any escape response, the threshold was estimated to be 26 g considering the sensitivity limit of this experiment.
Amorphous fractions of clotrimazole have a very low diffraction signal due to the small thickness of the film and are below the detection limit within this experiment.
Another limit of this experiment includes the lack of preoperative data for von Frey testing; instead, data for naïve littermate controls were used.
On the basis of these observations and under the assumption that continuous exponential growth occurs at a rate equivalent to that for R6 (generation time, 34 min), the proportion of cells carrying the duplication would drop below the detection limit of this experiment (5%) after 2.5 to 7 days.
Water (added to saturation every 2 3 days), nutrients (20∶20∶20 NPK added at onset), and light were not limiting in this experiment.
However, within the limits of this experiment, we could not achieve a higher cooling rate, and thus the freezing of the core lipids was limited by the cooling rate, while the intrinsic rate limit might actually be faster.
The amount of CSF that could be collected per animal per time point was limiting in this experiment because of practical issues with diabetic rats; therefore, not all the indicators were measured at some time points.
This demonstrates that LA of the concretion samples generates particles of uniform chemical composition, at least to the, albeit limited, resolution of this experiment.
This might because the DOC can be released from PBS automatically by enzyme degradation according to the nitrate loading and DOC was not a limiting factor in this experiment.
