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"And I don't limit the reading".
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In some applications the physical constraints (e.g., radiated power from the reader) of the RFID-system set the limit of functionality (e.g., limits the reading range).
Nevertheless, this limited the reading of both scoring methods similarly.
By limiting the reading frame overlap to less than half of the minimum length of the uORFs being investigated in this study, we hoped to minimise any biasing effect caused by this mechanism.
(We limited the read-depth to less than 1100× to avoid transposable elements, but in principle these could also be disambiguated).
"In scientific journals the question is no longer whether humans are to blame, but how little time we have got left to limit the damage," read the editorial, which was widely distributed in a campaign led by Britain's Guardian newspaper.
We use the clone orderings to limit the computation of read overlaps.
There are additional issues related to cost, that robotic sample handling might limit the type of read-out assay, the requirement for easily transfectable cell-lines, and even the possibility that an SS/SL interaction might be cell-type specific.
To identify gene expression differences between males and females we limit the analyses to reads that map to exon sequences, as opposed to the full gene sequence that contains introns.
Small inserts were essential for these techniques, since longer clones will often include repetitive elements along with genes and thereby be excluded; however, the small sizes limit the ability of the read pairs to link contigs into scaffolds.
I limit the time I spend reading emails or thinking about work when office hours are over.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com