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Samples were randomly assigned to the arrays within a slide, with all samples (time points) for a single animal hybridised onto the arrays on a single slide to limit technical variation.
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Conceptually, this source of variation is a lower bound on the technical variation which can be partitioned from the residual technical variation.
While there have been successful attempts to corroborate gene array data in independent patient cohorts [ 3], the limited dynamic range and considerable technical variation (large batch effects) of hybridization-based arrays will very likely continue to limit their utility for medical purposes.
The clustering of samples of the same tissue suggested limited biological and technical variations, indicating data consistency.
Given the limits of differential methylation detection above the technical variation of pyrosequencing, regions for validation were selected among the greatest absolute shifts in methylation associated with GDM, with a p value <0.001.
However, the uncovered subtypes may also reflect idiosyncrasies, technical variation, representational and measurement biases that limit the ability of the model and results to generalize to other studies or platforms.
Another possible explanation for this variability is technical variation, because the assay could have been at the limit of its sensitivity.
For both lines, the level of protein expression variation was in the range of technical variation.
Thus, technical variation can substantially affect the interpretation of microarrays.
Therefore, this CV is an assessment of technical variation.
So, it is not clear whether all the variation removed was technical or whether all the technical variation was removed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com