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For both strains, the detection limit of the array was 10 pg genomic DNA per assay; this amount of DNA was approximately equal to 270 cells (37 fg of DNA per cell of Candida albicans) [ 13].
The frequency of CNVRs in a breed is however not an exact measurement since it can depend on e.g. the discovery procedure, filtering criteria and whether the size of the CNVR is close to the detection limit of the array.
Cytokine array analysis did not reveal any salient increases in cytokines in CM-PA compared to CM-PO, however the array is limited and it is also possible that levels below the detection limit of the array, or proteins not analyzed, confer polarization.
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Other zinc transporters did not appear in the gene lists of significantly regulated transcripts, either because their expression was below the detection limits of the array or they did not pass the stringent statistical criteria associated with FDR associated with parallel analysis of >16 K genes.
The detection limit of the sensor array was ≤1.0 ng/mL for most analytes.
In the next section we present other examples showing that the hook method reasonably estimates the detection limit of the particular array in terms of present and absent calls.
Eight clones gave signals similar to background, suggesting that the concentration of these clones in the blood sample is below the detection limit of the T-array.
For one, the scarce number of samples included in the microarray analysis may limit the validity of the array results.
The second file contains the miRNAs confidently identified using the internal control bioB-3 signal intensity as a threshold for the limit of detection of the array.
We note that the protein array may have disadvantages, such as the limited sample number (only one) for each type of tissue, which greatly affects the statistical reliability of any observation, and the loss of signal intensity after stripping and re-probing, which limits the use of the array for further probing with other antibodies.
For determination of CNV frequencies, only those CNVs that are of the recurrent size, as determined within the limits of resolution of the array used, are counted; any CNVs that do not include the entire region or extend into surrounding regions are excluded.
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