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Exact(17)
The lower limbs were removed as described above and the femur and tibia shafts were immediately placed in a sterile hood and flushed with MSC medium (alpha MEM supplemented with 1% alpha glutamine, 1% antibiotic, 10% Hyclone SVF) containing heparin (5 IU/mL .This solution was passed through a 60 μm filter and centrifuged for 10 min at 1500 rpm.
Tail and limbs were removed.
The tumour-affected limbs were removed along with lungs, heart, intestines and skin.
For histological assessment, mice were sacrificed at day 42 post first CII immunization, and both hind limbs were removed.
C57BL/6 mice were killed by CO2 asphyxia and their limbs were removed for bone marrow isolation.
Front and hind limbs were removed and dissected from euthanized mice at the conclusion of the experiment.
Similar(43)
Immediately after euthanasia of the animals, the skin of the limbs was removed and the bones procured.
Both forelimb and hind limb were removed from the right side carcass of F2 animals and dissected from the limb.
After slaughter, both the forelimb and hind limb were removed from the left side of the carcass of the F2 animals.
The operation was then commenced, and the limb was removed in what seemed to us a marvellously short space of time – certainly less than a minute; the patient remaining perfectly still and motionless.
For skin preparation, the hair of the hind limb was removed, and the skin was cleaned.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com