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pombe stranded RNA-seq experiment, there were 507 instances reported by CodingQuarry of likely transcript fusions.
All chimeras whose most likely transcript was either NM or XM were deemed to be a small RNA-mRNA chimera.
For the chimeric reads, the most likely transcript was deemed to be the transcript which was most abundant in the data.
Since ASPic is also able to detect the most likely transcript variants for a gene, we propose here two simple extensions to ASPic-GeneID that allow the prediction of alternative splicing transcripts.
In order to estimate the most likely transcript abundance distribution for the single cells analysed here, we varied the scale and shape parameters of the distribution to better fit the experimental distribution observed in the 'half-vs-half' microarray data.
The path with the smallest number of differences from the read sequence, representing the likely transcript of origin, is then used to correct k-mers in the original read.
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Most likely, transcripts of this size were synthesized in vivo given the RT-PCR results obtained and the locations of the primers used on the gene sequence.
These results convincingly show that a substantial proportion of nTARs are most likely transcripts of protein-encoding genes rather than pseudogenes or regulatory RNAs.
All four packages are based on constructing, simplifying, and resolving de Bruijn graphs to extract likely transcripts (see [ 8] for a general introduction).
High-resolution tiling microarrays have been used to generate predictions of likely transcripts, many of which mapped to intergenic and intronic regions of the genome that were not previously annotated [ 22- 24].
Although our experimental design did not control for stochastic effects (genetic drift) and the presence of false positives is likely, transcripts affected by these differential SNPs represent strong candidates for further functional validation studies.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com