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Two summary reports were created once the most likely alignment for each read was identified by BFAST.
Beside clearly aligned paired-end reads, ambiguously aligned paired-end reads (see e.g. Fullwood et al., 2009; Hall and Quinlan, 2012; Maher et al., 2009 for details on these concepts) are also considered and the most likely alignment position of such reads is calculated.
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This may occur for the repeat-rich human genome and equally likely alignments can still represent contaminating sequences.
In any case where there are two or more equally likely alignments (multiple locations a query can map to), the mapping quality is zero.
These results indicated that the fibrin fibrils were likely undergoing alignment and the formation of bundle-like structures during the initial tensile strain up to 50%.
Since a significant proportion of metagenomic data routinely falls into this hard case category, it is likely that alignment behavior of peptides from hard case proteins could confound quantification when using all alignment information of peptides in conjunction with frequency-based weighting.
Moreover they excluded all heterozygous sites, as C. reinhardtii is haploid and heterozygous sites likely represent alignment or sequencing errors.
All have homologous regions in the chloroplast genome and the high DOC very likely represented alignment of both mitochondrial and chloroplast transcripts since total RNA was sequenced.
This approach provided a unique most likely orthologous alignment for 44,493 turkey BACs along the framework chicken sequence (Additional file 2: Table S2).
Note that this method can calculate the most likely structural alignment and its common secondary structure, sacrificing extremely huge computational time compared with M1, M2 and the proposed method.
We also found few mutations from either cancer set in the PI3_PI4_kinase domain, however, the C-terminal region of the domain is believed to be partially disordered [ 32], likely preventing alignment of the domain model to that region.
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