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However, whether selection imposed on one transcription cassette effects the likelihood of expression from a second, independent transcription cassette within the same vector has been less well studied.
The presence of AD pathology may reduce the likelihood of expression of the typical DLB phenotype [ 52, 53].
Next, we assigned a score to each TAR that ranks the TARs in order by likelihood of expression.
These mechanisms include strong selection against translation errors, causing slower evolution of early replicated genes because of the greater likelihood of expression, and a benefit of early replication of essential genes to avoid irreparable damage from the mutagenic environment and maintain their function.
The first is that replication timing still produces sufficient variation in gene dosage to influence the likelihood of expression and strength of purifying selection, as has been demonstrated in bacterial genomes of various compositions (Sharp and Li 1987; Sharp et al. 1989; Chen et al. 2004; Couturier and Rocha 2006; Rasmussen et al. 2007; Drummond and Wilke 2008; Cooper et al. 2010).
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Odds ratios express relative likelihood of SRE expression.
The expression of infant difficult behaviors at the age of 3 months increased the likelihood of the expression of these difficulties at 6 months by more than 5 times.
All transgenic constructs were expressed using the same backbone, thereby reducing the likelihood of differential expression in distinct cell types.
Interestingly, those wildtype genes showing no expression indeed mostly exhibit a relatively low CAI of ≤0.78 (Table 1), whereas all optimized genes mediating high-level expression have a CAI value close to 1, suggesting that the CAI might serve to predict the likelihood of successful expression in mammalian cells.
The regression model significantly predicts likelihood of SRE expression (χ = 274.52, df = 6, p < 0.005).
According to our binomial logistic regression model, the timing of bolting was a strong predictor of likelihood of SRE expression (χ6 = 274.52, p < 0.005; Table 1).
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