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Interestingly, although P-type like accessions were not discovered in the investigated germplasm, another genetic substructure with no correlation to geographic origin was evident within B. vulgaris, with three accessions (4, 8, 27) forming a distinct group.
Similar(59)
In addition, several hybrid-like accessions that combined both Chinese and Thailand parentage were observed at K=3.
The match between the tree position and the Structure population attributions was perfect for the indica and japonica accessions while the aus/ boro- and sadri/ basmati-like accessions were mostly classified as admixed, with a few aus/ boro-like accessions classified as indica.
Another dieback resistance gene was discovered in the primitive romaine-like accession PI491224 [ 6].
Whole-genome sequencing revealed that the supposedly trisomic stock is an introgression line, with one fifth of its genome coming from an Estland/Estonia (Est -like accEst -like
The results obtained for the different tissues were analyzed and standardized to the mRNA levels of poplar ACTINII-like (Accession number: EF145577), which shows stable expression.
The poplar ACTINII-like (accession: EF145577) and UBIQUITIN genes, which are constitutively expressed in performed with semi-quantitative real time RT-PCR analysis were selected as the internal control to which transcript abundance was normalized (Additional file 7: Figure S5).
These scenarios include commonly needed tasks like accession-independent linking up of a sequence to its most closely related entries in well curated databases such as GenBank (Benson et al., 2011), UniProt [(The Universal Protein Resource (UniProt) in 2010, 2010] or diverse pathway and interaction databases.
RDG2A was most similar (47 52%) over its whole length to five rice disease resistance-like proteins (accessions BAD08990, EEE69085, EEC83970, BAD0894, and BAF24312; Figure 5) encoded by genes clustered in a 2.97 Mbp region of rice chromosome 8 (nt. 25,872,241 to 28,845,527 of AP008214), which is not co-linear with the barley Rdg2a interval [7].
Publicly available OR-like EST accessions from NCBI correspond to ESTs detected using 454 sequencing of olfactory organ cDNA with the exception of two, EG337313 and EG335699.
In this work, we have cloned and characterised the AP2-like gene from accessions of Hordeum chilense and Hordeum vulgare, wild and domesticated barley, respectively, and compared with other AP2 homoeologous genes, including the Q gene in wheat.
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Justyna Jupowicz-Kozak
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