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A rip panel is an elongated section of the balloon that is lightly fixed in place and can be quickly ripped or pulled open at the moment of landing.
Cells were then lightly fixed in 0.5% formaldehyde/Hank's Buffered Salt Solution.
For immunohistochemistry, posterior eyecups were lightly fixed in 4% (w/v) paraformaldehyde in 0.1 M phosphate buffer (PB) (pH 7.4) for 15 30 minutes.
This labeling of rod terminals is unlikely to be a fixation artifact, since the sections were lightly fixed and labeling was specific to synaptic terminals.
In order to examine mitochondrial protein solubility in situ, we lightly fixed cells prior to extraction in low concentrations of non-ionic detergent, followed by processing for electron microscopy.
Live neuronal cultures were briefly incubated (10 min) with antibodies directed against extracellular N-termini of GFP (10 µg/mL in conditioned medium) to specifically label surface receptors, then lightly fixed for 5 min in 4% paraformaldehyde (non-permeabilizing conditions).
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ChIP usually involves lightly fixing cells of interest, usually with formaldehyde, to cross-link proteins and DNA.
If the Republicans are wise, they will choose a politician who travels more lightly, eyes fixed on the future.
To compare phagocytosis of C. albicans white and opaque cells, C. albicans cells were co-incubated with S2 cells and after variable time intervals the samples were lightly formaldehyde fixed, and phagocytosis was scored using immunofluoresence.
Fig. 4A shows abundant membrane localization of WT-ACE in lightly fixed-non-permeabilized cells, whereas we did not observe membrane localization of mutant Q1069R-ACE under the same conditions and detector settings (Fig. 4D).
For the histology analysis (Cases 2, 3), tissue samples were fixed in formalin, lightly decalcified with nitrogen oxide when necessary, trimmed and paraffin-embedded.
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