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As measure for irradiant light we used the spectrum of standard daylight D65, as an earlier study showed that the degree of discriminable variability was mostly unaffected by differences in illuminants [39].
The spectral sensitivities were measured with unpolarised light: we used a spectral scan method where a photoreceptor is clamped to a pre-selected DC potential by adjusting the light flux as we scanned from 300 to 730 nm in 10 nm steps with a monochromator.
In order to test whether Bmal1 brain knockouts entrain to light, we used a skeleton photoperiod (LDLD 1 10 1 12, Figure 3A E).
As diet is the only source of vitamin D for rodents (without a contribution from ultraviolet light), we used a vitamin D-deficient diet to alter vitamin D status.
To determine the kinetics of intracellular c-di-GMP accumulation in response to NIRW light, we used MG1655[DE3] expressing the bphS-bphO-yhjH operon with the RBS3 upstream of yhjH.
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Instead of a single real valued number representing the intensity of light, we use a complex valued number, which represents the amplitude and phase of the light.
The wavelength of the light we use to measure the vibrations is just a little bit larger than the size of the device.
We used light loggers that were first designed to geolocate birds' migratory patterns, but because they can detect the presence or absence of light, we use them to see if the squirrels are in their burrows or not.
To control the light, we use small liquid crystal devices that work like computer monitors but are much smaller.
"We don't have the ability and cannot afford to pay more money for each light we use," says Rahim, whose wife and nine children share a home with his brother, sister-in-law and their nine children.
For infection in the light, we use the same values of parameters Vr, Kmr, tr and ε, and found that λ = 1,027,800 (dNTP h−1 cell−1) gave a reasonable description of phage genome replication (Fig. 3).
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