Eyes were then dissected under light microscope to select the tyrosinase-positive portions of the eyecups.
One-micrometer thick sections were cut, stained with 1% methylene blue and viewed by light microscopy to select representative areas.
Sections were stained with toluidine blue, placed on glass slides, and examined by light microscopy to select appropriate areas for ultrathin preparation.
Each supernatant was then examined under light microscopy to select the fraction containing the higher proportion of villi with crypts still attached.
Semi-thin sections were cut with an ultramicrotome (Leica EM EC7), stained with 1% toluidine blue and screened by light microscopy to select areas in which the myelin fibers were perpendicular to the cutting plane.
Sections, 1 μm thick, were cut on a Reichert OMU4 ultramicrotome (Leica Microsystems), stained with toluidine blue and viewed in a light microscope to select suitable areas for investigation.
The samples were postfixed in 1% osmium tetroxide (OsO4), embedded in Epon, sliced into 1-μm-thick sections, stained with toluidine blue and viewed under a light microscope to select suitable areas for investigation.
When using lights be sure to select the right wattage of light, a 40 watt will do nicely on a 10 gallon (37.9 L) tank.
The next step involves scanning over the SEBS film surface to confirm the suitability for AFM tip hammering, and the sample surface is scanned in light tapping mode to select a large area suitable for hammering.
Firstly, the heavy chain variable gene of the Guy's 13 construct was introduced into a naïve human light chain phage display library to select human light chains that, in combination with the murine heavy chain, showed binding specificity for the SAI/II antigen.
