To attain a uniform distribution of light to the cells, we used a suspension of silica nanoparticles that scattered the light supplied by the light bulbs (2 × 2000 W) from the opposite square sides of the photobioreactor.
Because of the vivid light supplied by the Mediterranean climate his palette significantly brightened.
Arabidopsis thaliana plants for RIL development were grown under continuous light, supplied by Osram Fluora L36/W77 neon bulbs with an intensity of 150 µmol s−1 m−2 at 20°C and 60% relative humidity in an environment-controlled growth room.
The wavelengths tested were 470, 660 and 730 nm, along with white light supplied by Philips Cool White Fluorescent Bulbs (Newark, NJ).
All plants were grown in the glasshouse in 1 L pots at 26-28°C 26-28°C6 h of light sundered by Philips Sun-T Agro 400- or 600-W sodium lights.
All cultures were incubated at 25°C under a 16-h light/8-h dark photoperiod with light supplied by a fluorescent cool-white light (50 μmol m-2s-1 photonynthetic photon flux density).
The average particle size and the polydispersity index (PDI) were measured by DLS (dynamic light scattering) using a Zetasizer ZN particle size analyzer (Malvern Instruments, Southborough, UK) equipped with vertically polarized light supplied by an argon-ion laser.
During the whole experiment, plants were illuminated by natural light, supplied with Philips® Green Power lamps (400 μmol.m–2.s–1 photosynthetically active radiation in the canopy) for 16 h per day, and subjected to a thermoperiod of 20°C (day) and 15°C (night).
The intensity of light supplied to the PBR was measured using a quantum sensor (SR. NO. Q40526 of QUANTUM, Model LI-1400, LI-COR biosciences, Lincoln, NE, USA) on the inner surface of each PBR.
The steps or seats are rows of concrete, with lighting supplied by six soaring steel pyramidal columns that flank them.
