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The localisation of MT in MCF7 and HCC1806 cells after treatment with 10 μ M CdCl2 for 24 h was determined by immunohistochemical staining using a polyclonal rabbit antibody to MT. Prior to CdCl2 treatment, only a light staining for MT was observed, mainly in the cytoplasm of both cell lines.
Light staining for 3β HSD has also been described in pig [ 44], sheep [ 45] and human [ 46] fetal ovaries.
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In red fruits little GUS activity was observed in the Cab19 GUS lines, except for some light staining in locular material.
PCR products were subjected to electrophoresis in 1.5% agarose gel and were observed under ultraviolet light after staining for 15 min with (0.5 g/mL) ethidium bromide.
The level of expression in the tumour cells was evaluated using a semiquantitative scoring system: 0 for absence of immunostaining, 1 for light staining, 2 for moderate staining, and 3 for heavy staining.
Prior to sectioning and staining for light microscopy, RHE tissues were fixed in 4% (v/v) paraformaldehyde in PBS (pH 7.4), dehydrated in ethanol and embedded in paraffin wax.
In many instances, IF and EM were not available, nor was staining for light chains.
The neoplasm tissues (4 mm2) were deparaffinated then gradually dehydrated, embedded in paraffin, the tissue sections (4 μm) were stained by H&E staining for light microscopy.
In more recent years, evaluation of cells and tissues for apoptosis has evolved towards staining for light microscopic and flow cytometric analysis.
Finally, stronger mesangial staining for lambda light chains than kappa light chains may point to IgAN [ 2].
While the 81A antibody against phosphorylated alpha-synuclein may also nonspecifically recognize neurofilament light polypeptide (also known as NF-L) [ 48], here staining for neurofilament light polypeptide did not colocalize with that for phosphorylated alpha-synuclein (Fig. 6m-x).
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