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Field researchers removed the eel through "light restraint of the seal and a slow steady pull to get the eel out," the program said on Facebook.
Each mouse was kept in a Ballman cage (Natsume Seisakusho, Tokyo, Japan) suitable for light restraint to keep the mice awake.
After closure of the abdomen, the animals were put into cages under light restraint and allowed to recover from the anesthesia.
Under light restraint, 16 areas of the denervated paw were stimulated with a pin, and responses were scored on a 3-point scale (0, no response; 1, light or inconsistent response; and 2, strong consistent withdrawal response).
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The atmosphere throughout is of handmade simplicity, local materials, vast light and restraint.
Subjects were placed in a light head restraint within the scanner to limit head movement during acquisition.
Light physical restraint is sometimes necessary at the beginning of induction and usually realized by holding hands away from the mask, and when possible, by the patient's accompanying person.
Each rat then underwent a 4-day conditioning procedure in which an active analgesic drug or its vehicle (unconditioned stimulus) was associated with the following cues (conditioned stimuli): novel testing room (environmental), vanilla scent cue (olfactory), dim incandescent lighting (visual), restraint procedure/injection (tactile), and time of day and injection-test latency (temporal).
Her sound suited this attitude — "Pure Heroine," her improbably savvy début album, from 2013, could have been called "Pure Restraint": light finger-snapping, lyrics sung in a hushed whisper, synth and drum lines slowed to an ice-cold pace.
Every seat has its own lap bar restraint, light gun, and speaker that provides audio throughout the ride.
Accordingly, for animals on the normal light cycle the restraint stress took place during their resting period (light phase).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com