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Bright light, particularly early-morning light, protects against depression.
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A significant increase in light protecting metabolites occur together with the induction of transcriptional processes involved in protection of cellular structures at this early phase.
The samples were divided into small aliquots, light protected and stored at −20°C.
This solution was aged in an amber bottle in light protected 4°C environment for a minimum of 72 h prior to use.
All chloroauric acid solutions were aged in individual amber bottles under 4°C and light protected for a minimum of 3 days prior to use.
This solution was aged in an amber bottle, and light protected in a 4°C environment for a minimum of 72 h prior to use.
There is only one studied formulation that guarantees the correct dose administering and stability after 3 weeks stored at 5 °C and light protected.
The Cy5 dye was added to the antibody while vortexing and further on incubated under permanent agitation for 1 h at RT (light protected).
Then, cells were incubated in a light protected incubator for 1hour at 37°C.
Sections were incubated overnight under light protected vacuum to allow an optimal fixation of brain sections on slides.
Subsequently, 0.55 M iodoacetamide was added to the mixture and incubated for 30 min at room temperature and light protected.
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