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After the actual corneal flattening with microwave energy, riboflavin drops are administered over the treatment area and ultraviolet light is administered to crosslink the corneal collagen to lock-in the corneal flattening.
Depending on whether the light is administered during the early or late subjective night, the animal either delays or advances its clock, respectively [13] [15].
In photodynamic therapy (PDT) a photosensitizer – a molecule that is activated by light – is administered and exposed to a light source.
In photothermal therapy, light is administered, absorbed by the particles and converted into heat sufficient to destroy cells in the local vicinity of the nanoparticle.
However, bright white light intensity of 250 lux has been found to be as effective as 10000 lux when the light is administered early in the morning [ 13, 21].
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Light was administered through a 4×5.5 cm frame placed 3 cm away from the eye.
Light was administered 30 min post photosensitizer administration.
Necrotic damage was more intense and fewer tumor cells remained, when higher energy (150 J/cm) NIR light was administered.
One animal was used as a control in which light was administered at 500 J/cm without administration of the photosensitizer (sham treatment).
Here, treatment with continuous light was carried out with the same drug and light dose as for the fractionated treatment (see Table 1, Group IV), except that the light was administered continuously over 30 min.
The light was administered at one of 8 randomly assigned times-of-day or night, but was provided at the same time-of-day or night across the 3 days for each individual.
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