Sentence examples for light imaging was from inspiring English sources
Only white light imaging was used with HDTV, and the images of the tumor area were digitally stored.
The agreement between visible light and autofluorescence ligament insertions had a mean Dice coefficient of 0.84 and the mean Dice coefficient of the interobserver variability for visible light imaging was 0.85.
All light imaging was done on a Leica DMIRE microscope using a Hamamatsu ORCA-ER camera and a 10× air lens with a numerical aperture of 0.30 or a 40× air lens with a numerical aperture of 0.60.
Bioluminescent light imaging was facilitated by the custom-built optical imaging system as described above.
Tumor diameter and bioluminescent light imaging were measured bi-weekly.
Light microscopy imaging was used to assess cellular morphology of macrophages incubated with or without brominated noscapine analogs [21].
Light microscopic imaging was performed with a digital camera (Spot II; Diagnostic Instruments, Sterling Heights, MI) on a Nikon E600 Eclipse epifluorescent microscope with x2 60 planapochromatic objectives and a standard FITC filter cube (FITC; EX 465 495 nm; DM 505 nm; BA 515 555 nm) and TRITC filter cube (TRITC; EX 528 553 nm; DM 565 nm; BA 600 660 nm).
For the nighttime measurements, larvae were quickly mounted under a dim red light and imaging was performed in complete darkness.
Light sheet imaging was performed with a Lightsheet Z.1 system (Carl Zeiss, Germany) equipped with a water immersion 20x detection objective lens (W Plan Apochromat, NA 1.0), dual sided 10x illumination objective lenses (LSMF, NA 0.2), a pco.edge scientific CMOS camera (PCO) and ZEN software.
A custom-made automated mirror switches mechanically between both cameras in about 220 ms. Images were acquired through a water dipping lens (20x, 1.0 NA) and excitation light for fluorescence imaging was generated by a Poly V monochromator (Till Vision; Gräfelfing, Germany).
During imaging experiments, transmitted light images were also captured to ensure that there were no significant changes in cell morphology.
