Otherwise, it is a simple method which does not require a sophisticated instrumentation, i.e. it requires an ordinary light microscope for examination of the stained preparations.
A good light source, such as the dental operatory light, is essential for examination of the oral soft tissues, alveolar ridges, palate, and any erupted or erupting teeth.
A progressive cropping was made using semithin sections counterstained with toluidine-blue for light microscopy examination (for more details see Supplementary Fig. 1 a, b ).
We describe a simple and economical technique to convert any standard light microscope suitable for examination of malaria films into a polarization microscope.
Using highly sensitive focal plane arrays, the images can be acquired under lighting conditions suitable for examination of paintings and even illuminated manuscripts [15, 18].
Classmates "openly joked about our periods" and "would moon each other in PE class"; it fostered the kind of openness that infuses Wong's stand-up, in which women's bodies, sexual appetites and insecurities aren't avoided but get held up to the light for a closer examination.
Paraffin sections 3 5 μm thick were stained with hematoxylin and eosin and with Masson's trichrome for connective tissue for light microscopic examination.
Paraffin-embedded sections, 4- μm thick, were used for light microscopy examination after haematoxylin eosin safran (HES) staining, and for immunohistochemistry. Immunohistochemical study of MUC1, MUC2, MUC5B, MUC5AC and MUC6 mucin expression was performed on all tumour samples.
Sections from the above blocks were used for preparation of haematoxylin and eosin stained sections for light microscopic examination, in situ hybridization (ISH), and immunohistochemistry (IHC).
